How can I supply some ascorbic acid, can you give me a web site?
Method of ascorbic acid biosynthesis( fermentation)
The direct precursor to the synthesis of vitamin C is 2-keto-L-caronic acid (2-keto-L-gulonicacid, 2-KGA). The last step in the production of vitamin C is molecular conversion. 2-keto-L-cauronic acid converts into vitamin C. There are at least 6 known 2-keto-L-archaeonic acid production pathways: 1 D-sorbitol pathway, 2 L-sorose pathway, 3 L-aditoic acid( or L-archaeonic acid), 4 2-ketto-D-glucononic acid pathway, 2, 5, 5, 2, 5-diketone-D-gluconic acid pathway, 6 pathway to produce 2-keto-L-archaeonic acid directly from glucose fermentation. Only the second synthetic pathway, known as the "two-step fermentation method," developed by China itself, has been commercialized, making it the best one.
D Sorbitol's substance production
The C-1 team is the only 6-carbon sugar that differs from sugar and sorbitol. The ethanol team on D-glucose C-1 is thus reduced to a sugary substance, D-sorbitol. In business processes, D-glucose is catalyzed to handle the stress, and the carbonyl group is reduced to an alcohol hydroxyl group in order to make D-sorbitol when using nickel as catalyst and hydrogen as reducing agent as reducing agent. At 75°C, add 50% sugar to the mixture. It is used to modify pH 8.4 using lime emulsifier, press into the hydrogenation reactor, include nickel catalyst, and then enter the reaction at 3.43103 kPa, 140°C, the end point of the reaction when hydrogen is no absorbed. If the pH of the reaction is not totally controlled by pH8.0-8.5, mannose, the glucose's variable isomerization product, may be reduced to form mannitol. After the effect, replace the precursor by settling the settlement. The supply is approximately 97% after the effect water is treated with ion exchange resin and activated carbon under reduced pressure. This is a colorless, clear, or slightly yellowish, fluid liquid.
2-keto-L-coaronic acid's ferment by bacteria
By two-step detoxifying, the two bacterial species created 2-keto-L-cauronic acid. The conversion of D alcohol to L-sorbitose and then to 2-keto-L-aronic ammonia was first done.
The hydroxyl group at the C-2 place is kept from changing during the first stage of fermentation, and bacterial transformation enables this process to be specific because only the hydroxyl group at the C-2 position is kept from changing during the first step, which is D-sorbitol to L-sorbitose. Black and glycolic acids rods are preferable because some acetobacilli can act as transformants. Accessed by seed expanded culture, access to the fermenter, corn pulp, foam, yeast cream, calcium carbonate, and other components, pH 5.0-5.2%, are primarily sorbitol, corn pulp, foam, foam, and other components. The ventilation ratio was 1 1 1 1 1 VVM, and the sorbitol concentration was controlled at 24% to 27%. When the focus drops below zero, the ferment broth determines sorpear, which causes the fermentation to stop for about 10 hours. More than 98% of D-sorbitol was converted to L-sorbitol. For the second fermentation stage, the ferment broth was prepared by sterilizing it for 20 minutes at a low temperature of 60°C.
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